Time lapse recording showing the development of neuron polarity in a hippocampal neuron growing in vitro. This recording captures not only the formation of the axon (the longest process, oriented down...

Colorized transmission electron micrograph of a platinum replica showing the cytoskeletal organization of stubby dendritic spines in extracted hippocampal neurons after 14 DIV. Unlabeled inset shows t...

Platinum replica illustrating the cytoskeletal organization of dendritic spines from extracted 14 DIV neurons. Thin spines associate with dendrites at the base (bottom) and with axons by the head (top...

Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top)...

This is Video S1 and it corresponds to Figure 2A. It shows that local blebbistatin micropipetting, but not DMSO, increases spine formation and extension (arrowheads). Equal volumes of blebbistatin and...

This is Video S2 which corresponds to still images in top row of Figure 2D. It shows the spine dynamics of control neurons. Time-lapse confocal imaging was performed on DIV14 hippocampal neurons co-ex...

This is Video S3,which corresponds to still images in bottom row of Fig. 2D. It shows that knocking down levels of Myosin II B (MIIB) increases spine dynamics. Spines from MIIB knockdown neurons exten...