Intracellular localization of GFP-Vps74 in a wild-type S. cerevisiae background grown at 26C. In wild-type cells incubated at 26C or 37C, GFP-Vps74 is localized to Golgi compartments and the cytosol. ...

Intracellular localization of GFP-GOLPH3 in S. cerevisiae frq1-1 temperature-conditional strain grown at permissive temperature of 26C (control for 37C temperature-shift). In frq1-1 mutant after a 30 ...

Kre2-GFP is efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing wild-type, untagged, Vps74. Control image for images showing that localization of Kre2-GFP is lost when mutant f...

Intracellular localization of GFP-Vps74 W88A, R97A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-Vps7...

Intracellular localization of GFP-GOLPH3 in BY4742 vps74∆ sac1∆. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-GOLPH3 localizes, in additio...

Intracellular localization of GFP-GOLPH3 R171A, R174A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-G...

Intracellular localization of GFP-GOLPH3 in S. cerevisiae pik1-83 temperature-conditional strain grown at permissive temperature of 26C (control for 37C temperature-shift). In pik1-83 mutant after a ...

Intracellular localization of GFP-GOLPH3 in S. cerevisiae pik1-83 temperature-conditional strain grown at restrictive temperature of 37C. In pik1-83 mutant after a 30 minute incubation at the restrict...

Kre2-GFP is not efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing Vps74 with mutations in the sulfate-binding pocket (K178A R181A). Compare with control image CIL# 13450. Thi...

Intracellular localization of GFP-GOLPH3 in BY4742 vps74∆. GFP-GOLPH3 is localized to Golgi compartments and the cytosol. Control image for images showing ectopic expression of GFP-GOLPH3 with mutat...