Wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) colocalizes primarily at the Golgi (Giantin) (red) with Helix Pomatia Lectin (HPL) (green) in unstimulated HeLa cells. HPL binds various glycans but the Tn ant...

Constitutively active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) colocalizes primarily at the Golgi (Giantin) (red) with Helix Pomatia Lectin (HPL) (green) in unstimulated HeLa cells. HPL binds various g...

The Golgi Apparatus. In mammalian cells, the very complex architecture of the membrane system makes understanding the interrelationship of the different organelles within the cell difficult. Their dyn...

COP-I beta1 (green) staining at the Golgi (GM130, a cis-Golgi marker) (red) in unstimulated HeLa cells. Helix Pomatia Lectin (HPL) (gray) staining is also shown. HPL binds various glycans but the Tn a...

Constituvely active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) blocks redistribution of Helix Pomatia Lectin (HPL) (green) from Golgi to ER in Src-deficient SYF fibroblasts cotransfected with active Src...

Intracellular localization of GFP-GOLPH3 in a wild-type S. cerevisiae background grown at 37C. In wild-type cells incubated at 26C or 37C, GFP-GOLPH localizes to intracellular membranes more robustly ...

Intracellular localization of GFP-GOLPH3 in BY4742 sac1∆ vps74∆. SAC1 encodes an integral membrane phosphoinositide phosphatase that is localized to the ER and Golgi. In the sac1∆ vps74∆ mutan...

Intracellular localization of GFP-Vps74 K178A, R181A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-Vp...

Intracellular localization of GFP-GOLPH3 W81A, R90A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-GOL...

COP-I gamma1 (green) staining at the Golgi (GM130, a cis-Golgi marker) (red) in unstimulated HeLa cells. Helix Pomatia Lectin (HPL) (gray) staining is also shown. HPL binds various glycans but the Tn ...