Localization of Abp1-RFP (red; left) and Abp140-3GFP (green; center). By expressing Abp1-mRFP and Abp140–3GFP to visualize endocytic vesicles and actin cables respectively, we observed that approxim...

Four general zones of f-actin movement are seen in primary cultures of newt lung epithelial cells microinjected with X-rhodamine actin. At the leading edge and throughout the lamellipodium, f-actin s...

Localization of Alexa Fluor-594-α factor labeled endosomes (red; center) and Abp1-GFP labeled endocytic patches (green; left) in an arp3-D11A cell. Although the patches do not move off the plasma mem...

Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Fast retrograde flow still occurs in the lamellipodium, but the width of the lamellipodium is re...

This sequence shows actin nucleation on the dorsal surface of an advancing lamellipodium. Small actin nucleations have been termed inductipodia in nerve growth cones, since they can be induced by late...

Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus keratocyte after unprotected extraction without PEG and phalloidin. Actin network at lamellipodial rear di...

Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell. Fast retrograde flow occurs in the lamellipodium and slow retrograde flow in the lamellum. The cell was microinjected with X-rhodamine-conjugated a...

Improved visualization of actin filament branching in lamellipodia. EM of keratocyte or fibroblast lamellipodial actin network after cytochalasin D treatment (0.2 μM for 30 min or 0.5 μM for 10 min)...

Fluorescently-labeled actin from chicken muscle. Actin is a protein involved in muscle contraction and cell movement. Purified actin can form loose bundles that self-organize into regular patterns...

This video shows stationary epidermal cells with copious retrograde flow of actin. Actin-myosin networks initiate at the cell periphery and flow towards the cell body. Even though actin-nucleation and...